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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Chemistry | 20–25% | - Carbohydrates, lipids, and enzymes - Electrolytes, acid-base, and toxicology |
| Urinalysis and Other Body Fluids | 5–10% | - Microscopic analysis and disease correlation - Physical and chemical testing |
| Immunology | 5–10% | - Principles of immunology - Serologic procedures and immune diseases |
| Microbiology | 15–20% | - Bacteriology and mycology procedures - Virology and parasitology testing |
| Hematology | 20–25% | - Laboratory testing and hemostasis - Physiology and disease states |
| Laboratory Operations | 5–10% | - Quality assessment and safety - Instrumentation and laboratory mathematics |
| Blood Banking | 15–20% | - Blood group systems and immunology - Transfusion practice and testing - Blood products and components |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. A patient is admitted to the hospital with acute chest pain, but which of the following enzymes will be elevated FIRST if the patient had an MI?
A) LD
B) AST
C) CK
D) ALT
2. Osteoblasts are generally seen in clusters while plasma cells are often more diffusely distributed. Osteoblasts have delicate cytoplasm, whereas plasma cells have dense basophilic cytoplasm with a perinuclear halo.
Hematology
The large cell shown in the illustration to the right is occasionally seen in the bone marrow and can be mistaken for a plasma cell - what is this cell called:
A) Megakaryoblast
B) Osteoblast
C) Stem cell
D) Myeloblast
3. G6PD deficiency causes an increased suseptibility to the oxidation of hemoglobin, which in turn, causes the precipitation of hemoglobin inside of the red blood cell.
Which one of the following conditions is associated with glucose-6-phosphate dehydrogenase (G6PD) deficiency?
A) Hemoglobins with low oxygen affinities
B) Precipitation of hemoglobin
C) Faulty heme synthesis
D) Microcytic red cells
4. A zone of inhibition is the area around an antibiotic-infused paper disk that does not show any bacterial growth. The antibiotic impregnated on the disk will diffuse into the agar in the area surrounding the disk. If the bacteria are sensitive to the antibiotic, they cannot grow near the disk. The size of the zone is proportional to how sensitive the organism is. If the organism is resistant to the antibiotic, it will grow very closely to the disk.
The size of the zone of suppressed growth on a sensitivity plate using sensitivity disks is referred to as the zone of:
A) beta hemolysis
B) inhibition
C) alpha hemolysis
D) susceptibility
E) gamma hemolysis
5. Name the comprehensive written safety plan detailing the proper use and storage of hazardous chemicals in the workplace.
A) OSHA
B) chemical hygiene plan
C) SOP
D) MSDS
Solutions:
| Question # 1 Answer: C | Question # 2 Answer: B | Question # 3 Answer: B | Question # 4 Answer: B | Question # 5 Answer: B |


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